Ex situ reproduction in Ambystoma granulosum and Ambystoma lermaense (Amphibia: Ambystomatidae).

Abstract

Amphibians are disappearing worldwide. Several species have been lost, and thousands are in danger of extinction. Of the threatened species, some can not be protected in their own natural habitat, therefore it is imperative to accomplish ex-situ conservation actions that assure its preservation. Ambystoma granulosum and Ambystoma lermaense, are species in high priority to be protected by mean of this conservation strategy, since they are endemic species of Mexico and with a concern conservation status. Both species have been little studied, therefore in this study we investigated reproductive aspects that are vital for its conservation, as reproduction in laboratory, and hormonal induction with human chorionic gonadotrophin (hCG). Our results showed not significant difference in the sex morphology for both species, except for the swollen vent in the cloacal region of males. The reproductive cycle is annual and synchronic in males and females, with a maximum of activity from May to September. We obtained clutches only from couples of A. granulosum without hormonal induction in variable numbers with an average of 664 ± 325(SD) eggs and a fecundity from 40 to 90%. We obtained nine clutches from couples of Ambystoma granulosum with hCG induction ranging between 17 and 1182 eggs. In couples of Ambystoma lermaense induced, were obtained 16 clutches, ranging between 110 and 1691 eggs. We analyzed possible causes for the variability in fertility of eggs obtained by induction.