¿Bacterias en arañas? Presencia de Wolbachia (Rickettsiales, Ehrlichiaceae) en arañas Synspermiata, incluyendo el primer registro para la familia Sicariidae (Araneae)
DOI:
https://doi.org/10.21829/azm.2025.4112720Keywords:
Endosimbiótico; infección; arañas violinistas; arañas patonas; MéxicoAbstract
La infección por la bacteria endosimbiótica Wolbachia (Ehrlichiaceae) se ha registrado en una amplia variedad de artrópodos, incluyendo arañas. Dentro del orden Araneae, existen reportes previos de infección por Wolbachia en arañas Synspermiata. En este trabajo, evaluamos la presencia de Wolbachia en los géneros Physocyclus (Pholcidae) y Loxosceles (Sicariidae) mediante estudios moleculares utilizando el marcador mitocondrial Citocromo c oxidasa 1 (CO1). Detectamos la presencia de esta bacteria en una especie de Physocyclus y en siete especies de Loxosceles de México. Nuestros hallazgos representan el quinto registro para la familia Pholcidae y los primeros registros para la familia Sicariidae siendo los primeros registros de la bacteria Wolbachia en arañas de México. Combinando estos resultados con trabajos previos, la infección por Wolbachia en arañas se ha registrado en 19 familias, 70 géneros y 122 especies. La familia Linyphiidae tiene el mayor número de especies reportadas con infecciones por Wolbachia, con 43 especies en 27 géneros. Dentro del clado Synspermiata, Wolbachia ha sido documentada en las familias: Dysderidae, Pholcidae, Telemidae, y Sicariidae, siendo esta última la que tiene la mayoría de los registros con siete especies.
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References
Ahmed, M. Z., Li, S. J., Xue, X., Yin, X. J., Ren, S. X., Jiggins, F. M., Qiu, B. L. (2015) The intracellular bacterium Wolbachia uses parasitoid wasps as phoretic vectors for efficient horizontal transmission. PLoS pathogens, 11(2), e1004672. https://doi.org/10.1371/journal.ppat.1004672 DOI: https://doi.org/10.1371/journal.ppat.1004672
Ahmed, M. Z., Breinholt, J. W., Kawahara, A. Y. (2016) Evidence for common horizontal transmission of Wolbachia among butterflies and moths. BMC evolutionary biology, 16, 1–16. https://doi.org/10.1186/s12862-016-0660-x DOI: https://doi.org/10.1186/s12862-016-0660-x
Astrin J.J., Stüben P.E. (2008) Phylogeny in cryptic weevils: molecules, morphology and new genera of western Palaearctic Cryptorhynchinae (Coleoptera: Curculionidae). Invertebrate Systematics 22 (5): 503–522. https://doi.org/10.1071/IS07057 DOI: https://doi.org/10.1071/IS07057
Baldo, L., Prendini, L., Corthals, A., Werren, J. H. (2007) Wolbachia are present in Southern African scorpions and cluster with supergroup F. Current Microbiology, 55, 367–373. https://doi.org/10.1007/s00284-007-9009-4 DOI: https://doi.org/10.1007/s00284-007-9009-4
Batista, P. D., Keddie, B. A., Dosdall, L. M., Harris, H. L. (2010) Phylogenetic placement and evidence for horizontal transfer of Wolbachia in Plutella xylostella (Lepidoptera: Plutellidae) and its parasitoid, Diadegma insulare (Hymenoptera: Ichneumonidae). The Canadian Entomologist, 142(1), 57–64. https://doi.org/10.4039/n09-801 DOI: https://doi.org/10.4039/n09-801
Chen, Y. (2016) Analyzing and visual programming internet of things and autonomous decentralized systems. Simulation Modelling Practice and Theory, 65, 1–10. https://doi.org/10.1016/j.simpat.2016.05.002 DOI: https://doi.org/10.1016/j.simpat.2016.05.002
Coddington, J. A., Levi, H. W. (1991) Systematics and evolution of spiders (Araneae). Annual review of ecology and systematics, 565–592. https://doi.org/10.1146/annurev.es.22.110191.003025 DOI: https://doi.org/10.1146/annurev.ecolsys.22.1.565
Correa, C. C., Ballard, J. W. O. (2016) Wolbachia associations with insects: winning or losing against a master manipulator. Frontiers in Ecology and Evolution, 3, 153. https://doi.org/10.3389/fevo.2015.00153 DOI: https://doi.org/10.3389/fevo.2015.00153
Cordaux R, Michel-Salzat A, Bouchon, D. (2001) Wolbachia infection in crustaceans: novel hosts and potential routes for horizontal transmission. Journal of Evolutionary Biology, 14, 237– 243. https://doi.org/10.1046/j.1420-9101.2001.00279.x DOI: https://doi.org/10.1046/j.1420-9101.2001.00279.x
Dunaj, S. J., Bettencourt, B. R., Garb, J. E., Brucker, R. M. (2020) Spider phylosymbiosis: divergence of widow spider species and their tissues’ microbiomes. BMC Evolutionary Biology, 20, 1–17. https://doi.org/10.1186/s12862-020-01664-x DOI: https://doi.org/10.1186/s12862-020-01664-x
Durkin, E. S., Cassidy, S. T., Gilbert, R., Richardson, E. A., Roth, A. M., Shablin, S., Keiser, C. N. (2021) Parasites of spiders: Their impacts on host behavior and ecology. The Journal of Arachnology, 49(3), 281–298. https://doi.org/10.1636/JoA-S-20-087 DOI: https://doi.org/10.1636/JoA-S-20-087
Fenn, K., Conlon, C., Jones, M., Quail, M. A., Holroyd, N. E., Parkhill, J., Blaxter, M. (2006) Phylogenetic relationships of the Wolbachia of nematodes and arthropods. PLoS pathogens, 2(10), e94. https://doi.org/10.1371/journal.ppat.0020094 DOI: https://doi.org/10.1371/journal.ppat.0020094
Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Marine Biology, 3, 294–299.
Goodacre, S. L., Martin, O. Y., Thomas, C. G., & Hewitt, G. M. (2006) Wolbachia and other endosymbiont infections in spiders. Molecular Ecology, 15(2), 517–527. https://doi.org/10.1111/j.1365-294x.2005.02802.x DOI: https://doi.org/10.1111/j.1365-294X.2005.02802.x
Goodacre, S. L. (2011) Endosymbiont infections in spiders. In Advances in Insect Physiology (Vol. 40, pp. 137-153). Academic Press. https://doi.org/10.1016/B978-0-12-387668-3.00003-9 DOI: https://doi.org/10.1016/B978-0-12-387668-3.00003-9
Hall, T, A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95–98.
Hertig, M. (1936) The rickettsia, Wolbachia pipientis (gen. et sp.n.) and associated inclusions of the mosquito, Culex pipiens. Parasitology, 28: 453–486 DOI: https://doi.org/10.1017/S0031182000022666
Hertig, M., Wolbach, S.B. (1924) Studies on rickettsia-like microorganisms in insects. Journal of Medical Research, 44: 329–374
Hilgenboecker, K., Hammerstein, P., Schlattmann, P., Telschow, A., Werren, J. H. (2008) How many species are infected with Wolbachia? a statistical analysis of current data. FEMS Microbiology Letters, 281(2), 215–220. https://10.1111/j.1574-6968.2008.01110.x DOI: https://doi.org/10.1111/j.1574-6968.2008.01110.x
Kittayapong, P., Jamnongluk, W., Thipaksorn, A., Milne, J. R., Sindhusake, C. (2003) Wolbachia infection complexity among insects in the tropical rice‐field community. Molecular Ecology, 12(4), 1049–1060. https://doi.org/10.1046/j.1365-294x.2003.01793.x DOI: https://doi.org/10.1046/j.1365-294X.2003.01793.x
Le Clec'h, W., Chevalier, F.D., Genty, L., Bertaux, J., Bouchon, D., Sicard, M. (2013) Cannibalism and predation as paths for horizontal passage of Wolbachia between terrestrial isopods. PLoS One 8:e60232. https://doi.org/10.1371/journal.pone.0060232 DOI: https://doi.org/10.1371/journal.pone.0060232
Li, S. J., Ahmed, M. Z., Lv, N., Shi, P. Q., Wang, X. M., Huang, J. L., Qiu, B. L. (2017) Plant–mediated horizontal transmission of Wolbachia between whiteflies. The ISME journal, 11(4), 1019–1028. https://doi.org/10.1038/ismej.2016.164 DOI: https://doi.org/10.1038/ismej.2016.164
Navarro-Rodríguez, C. I., Valdez-Mondragón, A. (2020) Description of a new species of Loxosceles Heineken & Lowe (Araneae, Sicariidae) recluse spiders from Hidalgo, Mexico, under integrative taxonomy: morphological and DNA barcoding data (CO1+ ITS2). European Journal of Taxonomy, 704, 1–30. https://doi.org/10.5852/ejt.2020.704 DOI: https://doi.org/10.5852/ejt.2020.704
Navarro-Rodríguez, C. I., Valdez-Mondragón, A. (2024) Violins we see, species we don’t… Species delimitation of the spider genus Loxosceles Heineken & Lowe (Araneae: Sicariidae) from North America using morphological and molecular evidence. Zootaxa, 5428(4), 527–548. https://doi.org/10.11646/zootaxa.5428.4.4 DOI: https://doi.org/10.11646/zootaxa.5428.4.4
Nolasco, S., Valdez-Mondragón, A. (2022) To be or not to be… Integrative taxonomy and species delimitation in the daddy long-legs spiders of the genus Physocyclus (Araneae, Pholcidae) using DNA barcoding and morphology. ZooKeys, 1135, 93. https://doi.org/10.3897/zookeys.1135.94628 DOI: https://doi.org/10.3897/zookeys.1135.94628
Rigaud, T., Juchault, P. (1995) Success and failure of horizontal transfers of feminizing Wolbachia endosymbionts in woodlice. Journal of Evolutionary Biology, 8(2), 249–255. DOI: https://doi.org/10.1046/j.1420-9101.1995.8020249.x
Rodriguero, M. S. (2013) Wolbachia, una pandemia con posibilidades. Revista de la Sociedad Entomológica Argentina, 72 (3–4), 117–137. http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802013000200001&lng=es&tlng=pt.
Rowley, S.M., Raven, R.J., McGraw, E.A. (2004) Wolbachia pipientis in Australian spiders. Current Microbiology, 49, 208– 214. https://doi.org/10.1007/s00284-004-4346-z DOI: https://doi.org/10.1007/s00284-004-4346-z
Rozen, S., Skaletsky, J, H. (2000) Primer3 on the www for general users and for biologist programmers. In: Krawertz, S., Misener, S. (eds). Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press, Totowa, N, J. pp 365–386. DOI: https://doi.org/10.1385/1-59259-192-2:365
Su, Q., Hu, G., Yun, Y., Peng, Y. (2019) Horizontal transmission of Wolbachia in Hylyphantes graminicola is more likely via intraspecies than interspecies transfer. Symbiosis, 79(2), 123–128. https://doi.org/10.1007/s13199-019-00623-5 DOI: https://doi.org/10.1007/s13199-019-00623-5
Valdez-Mondragón A., Navarro-Rodríguez C.I., Solís-Catalán K.P., Cortez-Roldán M.R., Juárez-Sánchez, A.R. (2019) Under an integrative taxonomic approach: the description of a new species of the genus Loxosceles (Araneae, Sicariidae) from Mexico City. ZooKeys, 892, 93–133. https://doi.org/10.3897/zookeys.892.39558 DOI: https://doi.org/10.3897/zookeys.892.39558.figure70
Vanthournout, B., Swaegers, J., Hendrickx, F. (2011) Spiders do not escape reproductive manipulations by Wolbachia. BMC Evolutionary Biology, 11(1), 1–9. https://doi.org/10.1186/1471-2148-11-15 DOI: https://doi.org/10.1186/1471-2148-11-15
Vanthournout, B., Hendrickx, F. (2015) Endosymbiont dominated bacterial communities in a dwarf spider. PLoS One, 10 (2), e0117297. https://doi.org/10.1371/journal.pone.0117297 DOI: https://doi.org/10.1371/journal.pone.0117297
Vetter, R.S. (2015) The Brown Recluse Spider. Cornell University Press, Ithaca and London, Comstock Publishing Associates. 197 pp. DOI: https://doi.org/10.7591/9780801456169
Werren, J. H. (1997) Biology of Wolbachia. Annual review of entomology, 42(1), 587–609. https://doi.org/10.1146/annurev.ento.42.1.587 DOI: https://doi.org/10.1146/annurev.ento.42.1.587
World Spider Catalog. (2024) World Spider Catalog. Version 20.0. Natural History Museum Bern, online at http://wsc.nmbe.ch, accessed on 13/09/2024. https://doi.org/10.24436/2
Yang, X. H., Zhu, D. H., Liu, Z., Zhao, L., Su, C. Y. (2013) High levels of multiple infections, recombination and horizontal transmission of Wolbachia in the Andricus mukaigawae (Hymenoptera; Cynipidae) communities. PLoS One, 8(11), e78970. https://doi.org/10.1371/journal.pone.0078970 DOI: https://doi.org/10.1371/journal.pone.0078970
Yang, X. H., Gong, Y. H., Xu, X., Yin, H. Q., Zhu, D. H. (2021) The diversity, recombination and horizontal transmission of Wolbachia in spiders in China. Journal of Asia-Pacific Entomology, 24(3), 940–947. https://doi.org/10.1016/j.aspen.2021.08.003 DOI: https://doi.org/10.1016/j.aspen.2021.08.003
Zimmermann, B. L., Bouchon, D., Almerão, M. P., Araujo, P. B. (2015) Wolbachia in Neotropical terrestrial isopods. FEMS Microbiology Ecology, 91(4), fiv025. https://doi.org/10.1093/femsec/fiv025 DOI: https://doi.org/10.1093/femsec/fiv025
Zug, R., Koehncke, A., Hammerstein, P. (2012) Epidemiology in evolutionary time: the case of Wolbachia horizontal transmission between arthropod host species. Journal of Evolutionary Biology, 25(11), 2149–2160. https://doi.org/10.1111/j.1420-9101.2012.02601.x DOI: https://doi.org/10.1111/j.1420-9101.2012.02601.x
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