Can low concentration flufenoxuron treatment increase the pathogenicity or production of nucleopolyhedrovirus occlusion bodies in Spodoptera exigua (Hübner) or Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae)?
(English)
DOI:
https://doi.org/10.21829/azm.2023.3912627Keywords:
Insect growth regulator, peritrophic matrix, biological insecticide, occlusion bodies, BaculoviridaeAbstract
Los nucleopoliedrovirus (Baculoviridae) son patógenos letales que infectan de manera natural a las larvas de lepidópteros. El inhibidor de la síntesis de quitina flufenoxuron, ha sido reportado como un potenciador de las propiedades insecticidas de los cuerpos de oclusión (OBs) de nucleopoliedrovirus mediante la degradación de integridad de la matriz peritrófica de las larvas. En el presente estudio se ha examinado el efecto de un tratamiento de flufenoxuron sobre la susceptibilidad de larvas de quinto estadio de Spodoptera exigua (Hübner) y Spodoptera frugiperda (JE Smith) a sus nucleopoliedrovirus homólogos, SeMNPV y SfMNPV, respectivamente. Ensayos preliminares indicaron que una concentración de 1 mg/l de flufenoxuron resultó en la mortalidad de un 6.6 - 13.3% de larvas, el cual podría ser utilizado en combinación con un inóculo de OBs a concentraciones de 1.5 × 104 OBs/ml para SeMNPV o 1.3 × 10⁶ OBs/ml para SfMNPV. El tratamiento de larvas con flufenoxuron no incrementó significativamente la mortalidad por virus en S. exigua (16.7 - 17.2%) o S. frugiperda (28.9 - 34.4%), y no tuvo efecto sobre el promedio de producción de OBs en larvas muertas por virus en ninguna de las especies. La identidad de SeMNPV y SfMNPV fue confirmada para cada virus mediante los perfiles realizados con la digestión con endonucleasas de restricción. Estos hallazgos contrastan con los resultados de estudios previos en los que una concentración más alta de flufenoxuron (100 mg/l) potenciaba la mortalidad por virus en el gusano de seda Bombyx mori L., aunque por razones no muy claras no se reportaba una mortalidad del insecto debida sólo al tratamiento de flufenoxuron. Concluimos que el flufenoxuron (1 mg/l) no tuvo el efecto de potenciar la actividad insecticida del SeMNPV o SfMNPV en sus respectivos hospederos.
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