Changes in membrane carbohydrates distribution associated to epididymal sperm maturation during the prolonged sperm storage period of Corynorhinus mexicanus bat (Chiroptera: Vespertilionidae)

Ahiezer Rodríguez Tobón, Reyna Fierro, Miguel Angel León Galván, Adolfo Rosado, Edith Cortés Barberena, Edith Arenas Ríos

Resumen


The Corynorhinus mexicanus bat provides an interesting experimental model for the study of epididymal sperm maturation because after testicular regression, this bat stores sperm in epididymal cauda for several months. Earlier research conducted by our group suggested that sperm maturation in this specie must be completed in the caudal region of the epididymis, although the precise factor linked with this phenomenon is unknown. Therefore, the aim of this work was to analyze changes in the distribution of N-acetylglucosamine and/or sialic acid, Fucose and Mannose carbohydrates in different membrane domains of sperm cells as they change from the caput to the cauda of the epididymis, as well as, their changes in different dates of capture. The sperm cells present a redistribution of N-acetylglucosamine and/or sialic when they arrived in the caudal region (September 11), but after storage until October 22 the distribution of N-acetylglucosamine and/or sialic acid changed. Mannose residues were found predominantly towards the acrosome during their entry into and transit through the three regions of the epididymis. The flow cytometry assay indicated that fluorescence intensity due to the presence of of N-acetylglucosamine and/or sialic acid on the sperm decreases as the sperm pass through the epididymal duct and as storage time in the cauda goes on. The Mannose fluorescence intensity, decreased in corpus and cauda from September 24 to October 8, though no differences appeared on the latter date. The presence of Fucuse was corroborated only by flow cytometry. In conclusion, the carbohydrate distribution on sperm membrane can be considered as part of the process of epididymal sperm maturation and is associated with the phenomenon of prolonged sperm storage that is characteristic of this specie. This adaptation allows the males to synchronize with the period of receptivity of the females, and then, carry out the matings.


Palabras clave


bats; epididymal sperm maturation; sperm storage; membrane carbohydrates; lectins

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Referencias


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DOI: https://doi.org/10.21829/azm.2020.3612245

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Acta Zoológica Mexicana (nueva serie), 2020, Vol. 36, es una publicación electrónica continua de acceso abierto, editada por el Instituto de Ecología, A.C. (INECOL), Carretera antigua a Coatepec 351, Col. El Haya, Xalapa, Ver. C.P. 91073, Tel. (228) 842-1800, extensión 4112 http://azm.ojs.inecol.mx/index.php/azm. Editor responsable: Sergio Ibáñez-Bernal, sergio.ibanez@inecol.mx. Reservas de Derechos al Uso Exclusivo No. 04-2019-021314325500-203, versión on-line ISSN 2448-8445, [antes también impresa ISSN 0065-1737], con registros otorgados por el Instituto Nacional del Derecho de Autor. Responsable de la última actualización de este volumen, Sergio Ibáñez-Bernal, Carretera antigua a Coatepec, 351, Col. El Haya, Xalapa, Ver., C.P. 91073. Fecha de la última modificación: 09 de enero de 2020.

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